Mitochondrial DNA insertions into the nuclear chromosomes of the maize Mo17 inbred line [abstract]

Abstract only availableMitochondria contain their own DNA separate from the nucleus; however, most of their genes have been transferred to the nucleus over evolutionary time. The lateral transfer of DNA from the mitochondria to the nucleus appears to be a continuing process and is more frequent in plants than in animals. Our laboratory has documented extensive variation in the nuclear-mitochondrial sequences (NUMTs) among maize inbred lines using total mitochondrial DNA (mtDNA) as probes onto mitotic metaphase chromosomes, a technique known as fluorescence in situ hybridization (FISH). The mitochondrial genome has been cloned into 20 cosmids, which were used to examine the insertions of individual segments. The focus of the current study was to use FISH with the 20 individual mtDNA-containing cosmids to locate mtDNA within the nuclear chromosomes of the Mo17 inbred line of maize and to compare these locations with those of the B73 line. We studied Mo17 because this line and its derivatives are used in crosses with B73-derived lines to create the most commonly used corn hybrids. Fifteen NUMTs had been detected on the Mo17 chromosomes using a mixture of 19 mtDNA-containing cosmids. However, only nine of the 15 NUMTs were seen when applying individual mtDNA-containing cosmids, suggesting that the portions of nuclear DNA corresponding to the individual mtDNA-containing cosmids were too small to be detected until many cosmids were combined. A large NUMT was previously detected on the long arm of chromosome 9 by 14 of the 20 individually tested cosmids in the B73 inbred line. However, a NUMT present at the same site in Mo17 was detected by only 2 of the 20 cosmids (5 and 20). This suggests that the major insertion in B73 is recent and that it may have inserted by homologous recombination

Beacon Journey: Improving Patient Outcomes: Reducing Adverse Drug Events Using an Inter-Professional Team Approach

Literature states that the use of sedation regimens that include routine reversal of benzodiazepines or narcotic agents are not recommded. An inter-professional team concurred that planned reversals would no longer be the standard of practice for patients post post-procedural sedation. An inter-professional team analyzed the use of reversal agents, reviewed literature related to sedation and analgesia by non-anesthesiologists, and proposed recommendations for practice changes.https://digitalcommons.centracare.com/nursing_posters/1056/thumbnail.jp

A (trans)formação reflexiva para/com professores a partir do auto(re)conhecimento

A formação não se constrói por acumulação (de cursos, de conhecimentos ou de técnicas),mas sim através de um trabalho de reflexividade crítica sobre as práticas e de(re)construção permanente de uma identidade pessoal (Nóvoa). As discussões aqui apresentadas tem como base a referência anterior, pois tratamos da necessidade de se repensar a formação docente, considerando a necessidade de que ela venha a ser permanente, contínua, reflexiva e cooperativa. A partir do auto-reconhecimento, ciente do movimento interativo que faz diariamente, o sujeito professor tem oportunidade de refletir sobre a sua prática, com sinalização para as suas potencialidades. Tem-se, então, como objetivo neste texto discutir a possibilidade de transformação da prática docente, a partir da (trans)formação pessoal do sujeitoinfo:eu-repo/semantics/publishedVersio

Food environment, income and obesity: a multilevel analysis of a reality of women in Southern Brazil.

The objective of this study was to explore relationships between the neighborhood food environment and obesity in urban women living in São Leopoldo, Rio Grande do Sul State, Brazil. A cross-sectional survey was carried out. This study was conducted with 1,096 women. Structured interviews were conducted using a standard pre-tested questionnaire. Obesity was defined as body mass index (BMI) ≥ 30kg/m2. Circular buffers of 400m in radius were created based on the centroid of the women's houses who participated, in the 45 census tracts inhabited by them. Neighborhood food establishments were identified through systematic survey of all streets in the study areas and geographical coordinates of shops were collected. Establishments were evaluated using the NEMS tool. The prevalence of obesity was 33% among the women participants. After adjusting for individual variables, supermarkets and healthy food establishments were positively associated with obesity, PR = 1.05 (95%CI: 1.01-1.10), PR = 1.02 (95%CI: 1.00-1.04), respectively, while mean buffer income was negatively associated, PR = 0.64 (95%CI: 0.49-0.83). Neighborhood food environment factors were associated with obesity even after controlled for individual variables, as socioeconomic variables, behavioral and food purchase

Study of ordered hadron chains with the ATLAS detector

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A search for resonances decaying into a Higgs boson and a new particle X in the XH→qqbb final state with the ATLAS detector

A search for heavy resonances decaying into a Higgs boson ($H$) and a new particle ($X$) is reported, utilizing 36.1 fb$^{-1}$ of proton-proton collision data at $\sqrt{s} =$ 13 TeV collected during 2015 and 2016 with the ATLAS detector at the CERN Large Hadron Collider. The particle $X$ is assumed to decay to a pair of light quarks, and the fully hadronic final state $XH \rightarrow q\bar q'b\bar b$ is analysed. The search considers the regime of high $XH$ resonance masses, where the $X$ and $H$ bosons are both highly Lorentz-boosted and are each reconstructed using a single jet with large radius parameter. A two-dimensional phase space of $XH$ mass versus $X$ mass is scanned for evidence of a signal, over a range of $XH$ resonance mass values between 1 TeV and 4 TeV, and for $X$ particles with masses from 50 GeV to 1000 GeV. All search results are consistent with the expectations for the background due to Standard Model processes, and 95% CL upper limits are set, as a function of $XH$ and $X$ masses, on the production cross-section of the $XH\rightarrow q\bar q'b\bar b$ resonance

Search for dark matter produced in association with bottom or top quarks in √s = 13 TeV pp collisions with the ATLAS detector

A search for weakly interacting massive particle dark matter produced in association with bottom or top quarks is presented. Final states containing third-generation quarks and miss- ing transverse momentum are considered. The analysis uses 36.1 fb−1 of proton–proton collision data recorded by the ATLAS experiment at √s = 13 TeV in 2015 and 2016. No significant excess of events above the estimated backgrounds is observed. The results are in- terpreted in the framework of simplified models of spin-0 dark-matter mediators. For colour- neutral spin-0 mediators produced in association with top quarks and decaying into a pair of dark-matter particles, mediator masses below 50 GeV are excluded assuming a dark-matter candidate mass of 1 GeV and unitary couplings. For scalar and pseudoscalar mediators produced in association with bottom quarks, the search sets limits on the production cross- section of 300 times the predicted rate for mediators with masses between 10 and 50 GeV and assuming a dark-matter mass of 1 GeV and unitary coupling. Constraints on colour- charged scalar simplified models are also presented. Assuming a dark-matter particle mass of 35 GeV, mediator particles with mass below 1.1 TeV are excluded for couplings yielding a dark-matter relic density consistent with measurements

The Changing Landscape for Stroke\ua0Prevention in AF: Findings From the GLORIA-AF Registry Phase 2

Background GLORIA-AF (Global Registry on Long-Term Oral Antithrombotic Treatment in Patients with Atrial Fibrillation) is a prospective, global registry program describing antithrombotic treatment patterns in patients with newly diagnosed nonvalvular atrial fibrillation at risk of stroke. Phase 2 began when dabigatran, the first non\u2013vitamin K antagonist oral anticoagulant (NOAC), became available. Objectives This study sought to describe phase 2 baseline data and compare these with the pre-NOAC era collected during phase 1. Methods During phase 2, 15,641 consenting patients were enrolled (November 2011 to December 2014); 15,092 were eligible. This pre-specified cross-sectional analysis describes eligible patients\u2019 baseline characteristics. Atrial fibrillation disease characteristics, medical outcomes, and concomitant diseases and medications were collected. Data were analyzed using descriptive statistics. Results Of the total patients, 45.5% were female; median age was 71 (interquartile range: 64, 78) years. Patients were from Europe (47.1%), North America (22.5%), Asia (20.3%), Latin America (6.0%), and the Middle East/Africa (4.0%). Most had high stroke risk (CHA2DS2-VASc [Congestive heart failure, Hypertension, Age  6575 years, Diabetes mellitus, previous Stroke, Vascular disease, Age 65 to 74 years, Sex category] score  652; 86.1%); 13.9% had moderate risk (CHA2DS2-VASc = 1). Overall, 79.9% received oral anticoagulants, of whom 47.6% received NOAC and 32.3% vitamin K antagonists (VKA); 12.1% received antiplatelet agents; 7.8% received no antithrombotic treatment. For comparison, the proportion of phase 1 patients (of N = 1,063 all eligible) prescribed VKA was 32.8%, acetylsalicylic acid 41.7%, and no therapy 20.2%. In Europe in phase 2, treatment with NOAC was more common than VKA (52.3% and 37.8%, respectively); 6.0% of patients received antiplatelet treatment; and 3.8% received no antithrombotic treatment. In North America, 52.1%, 26.2%, and 14.0% of patients received NOAC, VKA, and antiplatelet drugs, respectively; 7.5% received no antithrombotic treatment. NOAC use was less common in Asia (27.7%), where 27.5% of patients received VKA, 25.0% antiplatelet drugs, and 19.8% no antithrombotic treatment. Conclusions The baseline data from GLORIA-AF phase 2 demonstrate that in newly diagnosed nonvalvular atrial fibrillation patients, NOAC have been highly adopted into practice, becoming more frequently prescribed than VKA in Europe and North America. Worldwide, however, a large proportion of patients remain undertreated, particularly in Asia and North America. (Global Registry on Long-Term Oral Antithrombotic Treatment in Patients With Atrial Fibrillation [GLORIA-AF]; NCT01468701